Comments on: Induced Pluripotent Stem Cells: A New Stem Cell Line with a Long History http://www.allthingsstemcell.com/2009/06/induced-pluripotent-stem-cells-a-new-stem-cell-line-with-a-long-history/ Tue, 11 May 2010 05:50:46 +0000 http://wordpress.org/?v=2.9.2 hourly 1 By: All Things Stem Cell » Cancer Vaccines: Using Embryonic Tissues and Stem Cells to Vaccinate Against Cancer http://www.allthingsstemcell.com/2009/06/induced-pluripotent-stem-cells-a-new-stem-cell-line-with-a-long-history/comment-page-1/#comment-1627 All Things Stem Cell » Cancer Vaccines: Using Embryonic Tissues and Stem Cells to Vaccinate Against Cancer Tue, 04 May 2010 07:03:42 +0000 http://www.allthingsstemcell.com/?p=211#comment-1627 [...] stem cells (hESCs) (which are stem cells isolated from the inner cell mass of a blastocyst) and induced pluripotent stem cells (iPSCs) (which are adult cells that have been reprogrammed to be hESC-like). These similarities [...] [...] stem cells (hESCs) (which are stem cells isolated from the inner cell mass of a blastocyst) and induced pluripotent stem cells (iPSCs) (which are adult cells that have been reprogrammed to be hESC-like). These similarities [...]

]]> By: admin http://www.allthingsstemcell.com/2009/06/induced-pluripotent-stem-cells-a-new-stem-cell-line-with-a-long-history/comment-page-1/#comment-1583 admin Fri, 12 Feb 2010 05:27:00 +0000 http://www.allthingsstemcell.com/?p=211#comment-1583 Hi Aditi, Thank you for your comment! The original 2007 iPSC papers referenced above have that information. See: Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. Induction of Pluripotent Stem Cells from Adult Human Fibroblasts. Cell. 2007. 131: 1-12. Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., Slukvin, I. I., and Thomson, J. A. Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007. 318(5858): 1917-1920. The factors were actually added to, not deleted from, the original adult cells that were reprogrammed into iPSCs. In Thomson's paper, they list nearly 100 factors that they made vectors for originally to test (this is in supplemental table 1). But, they then tested 14 of these genes in subsets and this is how they found the core 4 that worked best to reprogram the cells. I hope this helps explain it! The original papers are the best source to look at, though sometimes they can be confusing or leave out details. Thank you for reading and commenting! Hi Aditi,
Thank you for your comment! The original 2007 iPSC papers referenced above have that information. See:

Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. Induction of Pluripotent Stem Cells from Adult Human Fibroblasts. Cell. 2007. 131: 1-12.

Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., Slukvin, I. I., and Thomson, J. A. Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007. 318(5858): 1917-1920.

The factors were actually added to, not deleted from, the original adult cells that were reprogrammed into iPSCs. In Thomson’s paper, they list nearly 100 factors that they made vectors for originally to test (this is in supplemental table 1). But, they then tested 14 of these genes in subsets and this is how they found the core 4 that worked best to reprogram the cells. I hope this helps explain it! The original papers are the best source to look at, though sometimes they can be confusing or leave out details.

Thank you for reading and commenting!

]]> By: Aditi http://www.allthingsstemcell.com/2009/06/induced-pluripotent-stem-cells-a-new-stem-cell-line-with-a-long-history/comment-page-1/#comment-1580 Aditi Thu, 11 Feb 2010 09:30:23 +0000 http://www.allthingsstemcell.com/?p=211#comment-1580 Hi.... Great article! Do you know where i can find info about how they really came down to using these 4 factors? because i remember reading somewhere (and correct me if im wrong) that there are some 24 factors that need to be deleted to get the same effect and then they somehow narrowed it down to 4.... if anyone has a reference or link for these, kindly let me know! :) thanks in advance Hi….
Great article!
Do you know where i can find info about how they really came down to using these 4 factors? because i remember reading somewhere (and correct me if im wrong) that there are some 24 factors that need to be deleted to get the same effect and then they somehow narrowed it down to 4….
if anyone has a reference or link for these, kindly let me know! :)
thanks in advance

]]> By: All Things Stem Cell » Better Understanding Cancer and Induced Pluripotent Stem Cells Through Their Similarities http://www.allthingsstemcell.com/2009/06/induced-pluripotent-stem-cells-a-new-stem-cell-line-with-a-long-history/comment-page-1/#comment-1407 All Things Stem Cell » Better Understanding Cancer and Induced Pluripotent Stem Cells Through Their Similarities Mon, 14 Sep 2009 03:19:50 +0000 http://www.allthingsstemcell.com/?p=211#comment-1407 [...] many papers have come out that highlight connections between cancer and induced pluripotent stem cells (iPSCs), the latter of which was discussed previously. These papers hold many implications for not only iPSCs, but for our understanding of cancer as [...] [...] many papers have come out that highlight connections between cancer and induced pluripotent stem cells (iPSCs), the latter of which was discussed previously. These papers hold many implications for not only iPSCs, but for our understanding of cancer as [...]

]]> By: Andrew J. Bonham http://www.allthingsstemcell.com/2009/06/induced-pluripotent-stem-cells-a-new-stem-cell-line-with-a-long-history/comment-page-1/#comment-801 Andrew J. Bonham Wed, 10 Jun 2009 20:30:43 +0000 http://www.allthingsstemcell.com/?p=211#comment-801 Very interesting article... I find myself amazed at how much work one or two labs can churn out to advance our understanding-- Perhaps you could do a post on current Stem Cell centers around the world, and how much man-power and budget is being devoted to them? Thanks for starting this site! Very interesting article… I find myself amazed at how much work one or two labs can churn out to advance our understanding– Perhaps you could do a post on current Stem Cell centers around the world, and how much man-power and budget is being devoted to them?

Thanks for starting this site!

]]> By: Javin http://www.allthingsstemcell.com/2009/06/induced-pluripotent-stem-cells-a-new-stem-cell-line-with-a-long-history/comment-page-1/#comment-799 Javin Tue, 09 Jun 2009 18:28:55 +0000 http://www.allthingsstemcell.com/?p=211#comment-799 Great article! It's comprehensive yet easy to follow. Great article! It’s comprehensive yet easy to follow.

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